When a mission in Japan becomes a hostage situation, Sarah and Chuck must face the merciless Nicholas Quinn (guest star Angus Macfadyen) aboard a speeding Japanese bullet train. Meanwhile, the mission leaves Casey with an impossible decision.

Cast: Zachary Levi, Yvonne Strahovski, Joshua Gomez, Ryan McPartlin, Mark Christopher Lawrence, Vik Sahay, Scott Krinsky, Sarah Lancaster, Adam Baldwin, Steven Stone